Histone H3.1 (K9) pAb

貨號 Cat. No.

BS5522

規格 Size

100 μg

品牌 Brand

Bioworld

Description

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

Histone H3.1 (K9) antibody detects endogenous levels of Histone H3.1 protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200

Western Blot(WB)

Western blot (WB) analyzes of Histone H3.1 (K9) antibody in extracts from COLO205 cells.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of Histone H3.1 (K9) antibody in paraffin-embedded human malignant lymphoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.

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