Proteins and Peptides
Histone H2B Antibody (LS-B2446)
- 貨號 Cat. No.
- LS-B2446
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2446 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C24283. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Histone H2B |
| Host: | Histone H2B antibody was produced in Rabbit |
| Antigen Species: | Histone H2B antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | Histone H2B antibody was raised against kLH-conjugated, synthetic peptide APAPKAcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F. The immunizing sequence has 13 out of 14 identical amino acids in mouse, chicken and rat. |
| Antibody Specificity: | Recognizes human acetylated Histone 2B, Mr 15kD. Species sequence Homology: mouse, chicken and rat. |
| Antigen Modification: | Acetyl-Lys12 |
| Predicted Species Reactivity: | Chicken, Mouse, Rat |
| Purification: | Antiserum |
| Presentation: | Serum, 0.05% sodium azide, 30% glycerol. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2446 was determined to be 1:100. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:1000 - 1:10000) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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