Histone H2A.X (E145) pAb
- 貨號 Cat. No.
- BS5524
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Histone H2A.X (E145) antibody detects endogenous levels of Histone H2A.X protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analyzes of Histone H2A.X (E145) antibody in extracts from HeLa cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of Histone H2A.X (E145) antibody in paraffin-embedded human malignant lymphoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.
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