His6-USP7CD
- 貨號 Cat. No.
- E-518
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
His6-USP7CD
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
42 kDa
> 95% by SDS-PAGE
Catalog #: E-518
His6-USP7CD
Product Source:
human recombinant
Also known as: His6-USP7CD (HAUSP catalytic domain)
USP7 (Herpes-Associated Ubiquitin Specific Protease) is a nuclear protein that was initially identified as a novel p53-interacting protein. The enzyme deubiquitinates p53 thus stabilizing the levels of this key tumor suppressor, and inducing p53-dependent cell growth repression and apoptosis. USP7 also indirectly affects p53 stability by deubiquitinating HDMX, a regulator of HDM2. Full-length USP7 (1102 amino acids) contains an N-terminal p53 binding domain and 2 highly conserved USP active site domains. The C-terminal domain interacts with HSV-1 protein ICP0. This enzyme is able to cleave model substrates including linear Ub fusions, poly- ubiquitinated EBNA1 and poly-ubiquitinated p53. This recombinant protein has an N-terminal his tag & residues 213-548 representing the catalytic domain (CD) of USP7. Accession # NP_003461.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
MW:
42 kDa
Purity:
> 95% by SDS-PAGE