His6-Use1
- 貨號 Cat. No.
- E2-677
- 規格 Size
- 50 μg 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Use1
human recombinant
Product Information
50 μg 100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
42.6 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-677
His6-Use1
Product Source:
human recombinant
Use1 is a recently identified E2 conjugating enzyme which is specifically charged with ubiquitin by an alternate E1 activating enzyme Uba6 (E-307). Uba6 has a distinct preference and specificity for Use1 based on the regions specific to the C-terminal ubiquitin-fold domain. Use1 is not active with the canonical ubiquitin E1 (UBE1, E-305). After activating ubiquitin, Uba6 transfers ubiquitin to Use1 leading to the formation of a thiolester linkage with its catalytic site cysteine (Cys188). The ubiquitin can then be conjugated to substrate proteins via specific E3 ligase enzymes. This protein contains an N-terminal His6 tag.
Product Information
Quantity:
50 μg 100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
42.6 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.