His6-UFM1 Activating Enzyme (E1)
- 貨號 Cat. No.
- E-319
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
His6-UFM1 Activating Enzyme (E1)
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with lot number.
48 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is
Catalog #: E-319
His6-UFM1 Activating Enzyme (E1)
Product Source:
human recombinant
Also known as: UBA5
His6-UFM1 Activating Enzyme is the E1-like enzyme responsible for the activation of the ubiquitin-like modifier, Ufm1 (UL-500). The enzyme contains a conserved ThiF domain with a nearby cysteine catalytic residue (Cys250)and ATP-binding motif GXGXXG. This protein is highly conserved in humans and other higher eukaryotes, but not yeast.The enzyme utilizes ATP to adenylate the C-terminal glycine residue of Ufm1, forming a high-energy thiolester bond with the active site cysteine residue and the release of AMP. The second step is the trans-esterification reaction whereby Ufm1 is transferred to the E2-like protein Ufc1 (E2-675). This protein has an N-terminal His6-tag.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH8.0. Concentration varies with lot number.
MW:
48 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is