His6-Ubiquitin K6R

貨號 Cat. No.
UM-HK6R
規格 Size
1 mg
品牌 Brand
Boston Biochem

Description

His6-Ubiquitin K6R

Catalog #: UM-HK6R




His6-Ubiquitin K6R






Product Source: 

human recombinant



Mutation of lysine 6 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 6 linkages with other Ub molecules. Ub K6R can form an E1-catalyzed active thioester at the Cterminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and for determining if poly-Ub chains are K6 linked.


Product Information



Quantity: 

1 mg






Stock: 

Soluble and stable aqueous buffers up to 5 mg/ml.






MW: 

9.3 kDa






Purity: 

> 95% by PAGE






Supplied: 

This is a lyophilized powder




 


Use & Storage



Use: 

Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.






Storage: 

Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.




 

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