His6-Ube2S
- 貨號 Cat. No.
- E2-690
- 規格 Size
- 50 μg 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Ube2S
human recombinant
Product Information
50 μg 100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
25.87 kD
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-690
His6-Ube2S
Product Source:
human recombinant
Ube2S (E2-24K, E2-EPF) shares 38% identity with S.cerevisiae UBC4 with a conserved UBC domain that is characteristic of ubiquitin E2 conjugating enzymes. This E2 enzyme is over-expressed in common human cancers and targets von Hippel-Lindau (VHL) for proteosomal degradation, thereby stabilizing HIF-1α for Ub-mediated destruction. Overexpression of Ube2S in vitro and in vivo boosts tumor-cell proliferation, invasion and metastasis through effects on the pVHL-HIF pathway, and may thus be a novel molecular target for therapeutic intervention. Ube2S has also been identified as a K11-specific E2 for the anaphase-promoting complex (APC/C), and the E2 depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity. In vitro, UBE2S elongates ubiquitin chains initiated by the E2 enzymes UbcH10 and UbcH5, enhancing the degradation of APC/C substrates by the proteasome. This protein has an N-terminal His6-tag. Accession # NP_055316.2.
Product Information
Quantity:
50 μg 100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
MW:
25.87 kD
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.