His6-Ube2E3
- 貨號 Cat. No.
- E2-678
- 規格 Size
- 50 μg 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Ube2E3
human recombinant
Product Information
50 μg 100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
24.9 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-678
His6-Ube2E3
Product Source:
human recombinant
Ube2E3 is a Class III E2 protein with unique C- and N-terminal extension in addition to the conserved UBC domain. This enzyme shares over 90% identity with UbcH6, mouse UbcM2 and Drosophila UbcD2, but the protein N-termini are divergent. This E2 enzyme is active in conjugation reactions with various E3 ligase enzymes (BRCA1, CHIP), and is known to interact with two RING-Finger proteins, ARA54 and RNF8, via its UBC domain. This protein has an N-terminal His6-tag. (Accession # NP_006348.1)
Product Information
Quantity:
50 μg 100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
24.9 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.