His6-UbcH6, Dominant Negative
- 貨號 Cat. No.
- E2-632
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-UbcH6, Dominant Negative
human recombinant
Product Information
100 μg
X mg/ml (X μM) in PBS, 10% glycerol, 1 mM DTT, pH 7.2. Actual concentration will vary with specific lot number.
21 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-632
His6-UbcH6, Dominant Negative
Product Source:
human recombinant
UbcH6 mediates the selective degradation of short-lived and abnormal proteins. The enzyme has high similarity to UbcH5 E2 subfamily, but it does not participate in HECT-domain mediated events. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH6 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions. This protein has an N-terminal His6 tag.
Product Information
Quantity:
100 μg
Stock:
X mg/ml (X μM) in PBS, 10% glycerol, 1 mM DTT, pH 7.2. Actual concentration will vary with specific lot number.
MW:
21 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.