His6-UbcH3, Dominant Negative
- 貨號 Cat. No.
- E2-611
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-UbcH3, Dominant Negative
human recombinant
Product Information
100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
27 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-611
His6-UbcH3, Dominant Negative
Product Source:
human recombinant
UbcH3 plays an essential role in the progression of cells from the G1 to S phase of the cell division cycle. One pathway (requiring Cdc34) initiates DNA replication by degrading a CDK (cyclin-dependent kinase) inhibitor. The second pathway, involves the anaphase-promoting complex (APC) initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins. The active site of this enzyme has been chemically inactivated for use as a negative or competitive control.
Product Information
Quantity:
100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
MW:
27 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.