His6-SUMO-3
- 貨號 Cat. No.
- UL-763
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
His6-SUMO-3
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
11.4 kDa
> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-763
His6-SUMO-3
Product Source:
human recombinant
Also known as: His6-SUMO-3 (SMT3B)
The ubiquitin-like SUMO-3 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-3 is derived from the precursor pro-SUMO-3 (Accession # NM_006936). Human SUMO-3 shares 47% and 87% identity with SUMO-1 and SUMO-2 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
MW:
11.4 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Storage:
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.