His6-SENP2CD
- 貨號 Cat. No.
- E-710
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
His6-SENP2CD
human recombinant
Product Information
50 μg
X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
28.7 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-710
His6-SENP2CD
Product Source:
human recombinant
Also know as: SUMO- specific protease 2 catalytic domain
The covalent modification of proteins by SUMO is reversible and is mediated by SENP enzymes. SENPs cleave residues from the C-terminus of SUMO precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. These enzymes also reverse the process of SUMOylation by removing poly-SUMO conjugates from substrate proteins. SENP1 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitin or NEDD8. N-terminally tagged SENP1 contains the catalytic domain consisting of residues 415-643. Accession # NP_05536
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
MW:
28.7 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.