His6-S5a
- 貨號 Cat. No.
- SP-400
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-S5a
human recombinant
Product Information
100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 250 mM NaCl, 5% glycerol, 1 mM DTT. Actual concentration varies with lot number.
43 kDa
> 95% by SDS-PAGE
Use & Storage
Typical concentrations for in vitro assays are 1-5 μM depending on experimental conditions and detection method.
Store at -80°C. Avoid multiple freeze/thaw cycles.
≥ 96.2 % by HPLC.
Catalog #: SP-400
His6-S5a
Product Source:
human recombinant
S5a (Rpn10), a component of the 19S regulatory complex, functions as a receptor by binding to and recognizing poly-ubiquitinated proteins destined for 26S proteasome degradation. The protein recognizes ubiquitin chains and ubiquitin protein conjugates via two UIM (ubiquitin interacting motif) domains. S5a is a useful “universal” substrate to monitor E3 ligase activity, since it can be ubiquitinated by a wide variety of enzymes from different classes (RING-Finger, U-box, CHIP and HECT enzyme classes). S5a can be modified by E3 enzymes which catalyze homogeneous (K48 or K63) or heterogeneous Ub chain linkages. The mechanism of S5a ubiquitination is dependent on its association with Ub or Ub chains via its UIM domains, which brings it into close proximity with the E3 allowing for modification. The recognition of S5a by E3s is thus atypical and non-selective since these enzymes do not bind directly to degrons (degradation motifs) within this protein.
Product Information
Quantity:
100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 250 mM NaCl, 5% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
43 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical concentrations for in vitro assays are 1-5 μM depending on experimental conditions and detection method.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity:
≥ 96.2 % by HPLC.