His6-Pro-SUMO-1
- 貨號 Cat. No.
- UL-705
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Pro-SUMO-1
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
13.7 kDa
> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations are 10-50 μM depending on conditions.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-705
His6-Pro-SUMO-1
Product Source:
human recombinant
Also known as: His6-Pro-SUMO-1 (SMT3C)
All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-1 (101 amino acids) is the inactive precursor of SUMO-1 (97 amino acids) and is processed at the C-terminus by SUMO-1 specific proteases (SENPs). The resulting SUMO-1 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumolyation reactions or as a substrate for SENPs. This His6 tag is at the N-terminus. NCBI accession # NM_003352.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW:
13.7 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations are 10-50 μM depending on conditions.
Storage:
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.