His6-Pro-ISG15
- 貨號 Cat. No.
- UL-620
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Pro-ISG15
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific Lot #.
18.8 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-620
His6-Pro-ISG15
Product Source:
human recombinant
Also known as: UCRP
The ubiquitin-like ISG15 is conjugated to intracellular target proteins. This pathway is distinct from that of ubiquitination with different substrate specificities and interactions with ligating enzymes. Pro-ISG15 (165 amino acids) is the inactive precursor of ISG15 (158 amino acids). The precursor is processed at the C-terminus by an ISG15-specific protease UBP43 (also known as USP18). The mature form of ISG15 contains the conserved C-terminal di-glycine residues which are critical in activation and conjugation reactions. This protein can be used as a negative control in ISGylation reactions or as a substrate for UBP43.
Note: His6 tag is on the C-terminus of the protein.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific Lot #.
MW:
18.8 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.