His6-p53
- 貨號 Cat. No.
- SP-450
- 規格 Size
- 20 μg
- 品牌 Brand
- Boston Biochem
Description
His6-p53
human recombinant
Product Information
20 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 450 mM NaCl, 10% glycerol. Actual concentration varies with lot number.
45.6 kDa
> 95% by SDS-PAGE
Use & Storage
Typical concentrations for in vitro assays are 0.5-2.5 μM depending on experimental conditions and detection method.
Store at -80°C. Avoid multiple freeze/thaw cycles.
≥ 96.2 % by HPLC.
Catalog #: SP-450
His6-p53
Product Source:
human recombinant
Tumor suppressor protein p53, a nuclear transcription factor, plays an essential role in the regulation of cell cycle and is frequently mutated or inactivated in many cancers. Numerous post-translational modifications modulate p53 activity including ubiquitination, phosphorylation, acetylation and methylation. The stability of p53 is regulated via the ubiquitin-proteasome pathway (UPP). MDM2 is an oncogenic ubiquitin E3 ligase that ubiquitinates p53, inhibits its transcriptional activity and promotes its degradation. Other E3 ligases that promote the proteasome-mediated degradation of p53 include Pirh2, COP1 and p300. USP7 (HAUSP) stabilizes p53 by deubiquitination and induces p53-dependent cell growth repression and apoptosis. Additional factors such as p14ARF and MdmX also modulate p53 function via the UPP. This recombinant protein is N-terminally tagged and produced in E.coli. Accession # NP_000537.
Product Information
Quantity:
20 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 450 mM NaCl, 10% glycerol. Actual concentration varies with lot number.
MW:
45.6 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical concentrations for in vitro assays are 0.5-2.5 μM depending on experimental conditions and detection method.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity:
≥ 96.2 % by HPLC.