His6-BAP1
- 貨號 Cat. No.
- E-345
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
His6-BAP1
human recombinant
Product Information
50 μg
X mg/ml (X μM) 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
81 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-345
His6-BAP1
Product Source:
human recombinant
BAP1 (BRCA1 Associated Protein 1) interacts with the RING-FINGER domain of the E3 ligase BRCA1 (Breast Cancer1, early onset protein) which functions as a tumor suppressor in the BRCA1 growth control pathway. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs) and recombinant BAP1 has deubiquitinating activity in vitro. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. It has been demonstrated that BAP1 and BRCA1 associate in vivo and have overlapping sub-nuclear localization patterns. BAP1 appears to be a key regulator of the BRCA1 growth control pathway and has been proposed to be a novel candidate tumor suppressor. This recombinant protein is N-terminally tagged. Accession # NP_004647.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
MW:
81 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.