His6-Ataxin UIM
- 貨號 Cat. No.
- UBE-100
- 規格 Size
- 250 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Ataxin UIM
Product Information
250 μg
X mg/ml (X μM) 50 mM Hepes, 200 mM NaCl, 1 mM DTT pH 8.0
>95% by SDS-PAGE
Use & Storage
Use 50-100 μg of protein to detect 10-20 μg of purified K48-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.
Catalog #: UBE-100
His6-Ataxin UIM
Product Source:
human reconbinant
Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an catalytic N-terminal Josephin domain, three ubiquitin interacting motifs (UIMs), and a variable C-terminus with a polyglutamine stretch. Ataxin3 has deconjugating activity and functions as a mixed linkage, chain editing enzyme with preferential cleavage of K63 linkages in mixed chains. Ataxin3 also bind both K48-linked and K63-linked poly-Ub chains via its UIM domains and preferentially interacts with four or more ubiquitin units. This protein can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages. This protein is His-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies.
Product Information
Quantity:
250 μg
Stock:
X mg/ml (X μM) 50 mM Hepes, 200 mM NaCl, 1 mM DTT pH 8.0
Purity:
>95% by SDS-PAGE
Use & Storage
Use:
Use 50-100 μg of protein to detect 10-20 μg of purified K48-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.