His6-Ataxin-3
- 貨號 Cat. No.
- E-341
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Ataxin-3
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with lot number.
42 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-341
His6-Ataxin-3
Product Source:
human recombinant
His6-Ataxin-3 (MJD protein 1),
Defects in the ATXN3 gene are the cause of Machado-Joseph disease (MJD), also known as spinorerebellar ataxia 3. MJD is an autosomal dominant neurodegenerative disorder caused by an expansion of a (CAG)n repeat in the ATXN3 gene resulting in polymorphic protein forms in the C-terminal poly-glutamine region. Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an N-terminal Josephin domain, two ubiquitin interacting motifs (UIMs), and a variable C-terminus consisting of a polyglutamine stretch and tail. Ataxin-3 activity may have a role in MJD pathogenesis by influencing ubiquitin-dependent pathways that control protein folding and stability. This recombinant protein is N-terminally tagged. (Accession # NP_004984).
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with lot number.
MW:
42 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.