His6-Apg8p3 (MAPLC3a)
- 貨號 Cat. No.
- UL-430
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Apg8p3 (MAPLC3a)
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
16.3 kDa
> 95% by PAGE
Use & Storage
Typical concentration to support conjugation in vitro is 10-50 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-430
His6-Apg8p3 (MAPLC3a)
Product Source:
human recombinant
There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, including MAP-LC3a (Microtubule-Associated Protein Light Chain-3) which is part of the LC3 subfamily. The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in human, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. MAP-LC3 is the most abundant Apg8 homolog in autophagosomal membranes, and is thought to play a major role in mammalian autophagy. Accession # NP_115903.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
16.3 kDa
Purity:
> 95% by PAGE
Use & Storage
Use:
Typical concentration to support conjugation in vitro is 10-50 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.