His6-Apg8p2 (GATE16)
- 貨號 Cat. No.
- UL-420
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Apg8p2 (GATE16)
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
15.7 kDa
> 95% by PAGE
Use & Storage
Typical concentration to support conjugation in vitro is 10-50 μM depending on
conditions.
Store at -80°C. Avoid multiple freeze/ thaw cycles.
Catalog #: UL-420
His6-Apg8p2 (GATE16)
Product Source:
human recombinant
Also known as: GABARAPL2
There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, GATE-16 (Golgi-associated ATPase Enhancer of 16 kDa). The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in humans, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. GATE-16 is highly conserved from yeast to mammals, is constitutively expressed in secretory organs and localizes to the Golgi apparatus. The protein participates in membrane-trafficking events, and it has been proposed that GATE-16
modulates intra-Golgi transport by acting as an adaptor protein that couples NSF activity and SNARE activation. Accession # NP_009216.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
MW:
15.7 kDa
Purity:
> 95% by PAGE
Use & Storage
Use:
Typical concentration to support conjugation in vitro is 10-50 μM depending on
conditions.
Storage:
Store at -80°C. Avoid multiple freeze/ thaw cycles.