His6-Apg4b
- 貨號 Cat. No.
- E-400
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Apg4b
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT. Actual protein concentration will vary with specific Lot #.
46.5 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-400
His6-Apg4b
Product Source:
human recombinant
The Apg4b is a cysteine protease and a member of the autophagin protein family. This enzyme cleaves the C-terminal sequences of the precursor forms of Apg8 proteins (including GABARAP, GATE-16 and MAP-LC3), and regulates the lipidation and delipidation processes of these proteins. This specific cleavage reveals the conserved C-terminal glycine present in mature and active forms of the Apg8 proteins that are conjugated to phosphatidylethanolamine (PE) during autophagy.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT. Actual protein concentration will vary with specific Lot #.
MW:
46.5 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support hydrolysis of substrates in vitro is 10- 100 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.