His6-Apg3p
- 貨號 Cat. No.
- E2-670
- 規格 Size
- 50 μg 100 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Apg3p
human recombinant
Product Information
50 μg 100 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.
36.8 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-670
His6-Apg3p
Product Source:
human recombinant
Also known as: His6-Apg3p (Apg3L)
Apg3p is the E2-like enzyme necessary for the covalent conjugation of human Apg8 modifier proteins to lipid phosphatidylethanolamine (PE) and is essential in autophagosomal membrane formation. The Apg8 ubiquitin-like proteins (including GABARAP, GATE-16, MAP-LC3) are first activated by the E1-like activating enzyme Apg7p. Apg7p forms a complex with Apg3p which subsequently forms a glycyl thiolester bond with Apg8 proteins through its active site cysteine (C264). Accession # NP_071933.
Product Information
Quantity:
50 μg 100 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.
MW:
36.8 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.