His6-Apg Activating Enzyme ( Apg7L)
- 貨號 Cat. No.
- E-317
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
His6-Apg Activating Enzyme ( Apg7L)
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific lot number.
78.9 kDa
> 95% by SDS-PAGE
Use & Storage
Catalog #: E-317
His6-Apg Activating Enzyme ( Apg7L)
Product Source:
human recombinant
Autophagy is a process of bulk degradation of cytoplasmic components which are sequestered in double-membrane vesicles (autophagosomes) that deliver the contents to the lysosomal/vacuolar system for degradation. Apg7L is a homodimeric ubiquitin-activating E1-like enzyme essential for Apg8 and Apg12 conjugation that mediates membrane fusion in autophagy. This enzyme activates the C-terminal glycine of both ubiquitin-like proteins (UBLs) Apg8 and Apg12 in an ATP-dependent manner. Its activity is essential for the conjugation of Apg8 proteins to the lipid phosphatidylethanolamine (PE), and the conjugation of Apg12L to Apg5L, and is a critical component for the initiation of any in vitro conjugation reactions. Accession # NP_006386.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific lot number.
MW:
78.9 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.