His6-A20CD
- 貨號 Cat. No.
- E-344
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
His6-A20CD
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
46 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support hydrolysis of poly-ubiquitin chains (K48- or K63-linked) in vitro ranges from 1-5 μM depending on conditions and substrate.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-344
His6-A20CD
Product Source:
human recombinant
Also known as: His6-A20CD (TNFAIP3 catalytic domain)
A20 (TNFα-induced protein 3) is a cytoplasmic zinc finger protein that inhibits NFκB activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NFκB-dependent gene expression by interfering with RIP- or TRAF2- mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NFκB signaling. This catalytic domain (CD) has an N-terminal his tag and residues 1-371 of A20. Accession #
NP_006281.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
MW:
46 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support hydrolysis of poly-ubiquitin chains (K48- or K63-linked) in vitro ranges from 1-5 μM depending on conditions and substrate.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.