His Tagged Protein Purification Buffer Kit His蛋白質純化試劑

His Tagged Protein Purification Buffer Kit

(Cat. No. CH000 )

Introduction:

Immobilized metal ion affinity chromatography (IMAC) exploits the interaction between chelated transition metal ions and side-chains of certain amino acids (mainly histidine) on proteins. In general, Ni2+ is the preferred metal ion for purification of recombinant histidine-tagged proteins and imidazole is used for elution.

Imidazole at low concentrations is commonly used in the binding and wash buffer to minimize binding of unwanted host cell proteins. For the same reason, it is important to also include imidazole in the sample (generally, at the same concentration as in the wash buffer). At somewhat higher concentrations, imidazole may also decrease the binding of histidinetagged proteins. The imidazole concentration must therefore be optimized to ensure the best balance of high purity (low binding of unwanted proteins) and high yield (binding of all of the histidine-tagged protein). The concentration of imidazole that will give optimal purification results is protein-dependent, but 20 mM will work well for many proteins.

Contents of the kit: