His-Tag Antibody
- 貨號 Cat. No.
- 3998-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongHis-Tag/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /b6xHis synthetic peptide/p
pbAccession#: /bN/A/p
pbGene ID: /bN/A/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /ball/p
pbApplication: /bWB/IP/IC/p
pbApplication amp; Usage: /bWestern blotting (1 micro;g/ml), immunoprecipitation (40 micro;g/ml), and immunocytochemistry (2 micro;g/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bA variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /b6xHis synthetic peptide/p
pbAccession#: /bN/A/p
pbGene ID: /bN/A/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /ball/p
pbApplication: /bWB/IP/IC/p
pbApplication amp; Usage: /bWestern blotting (1 micro;g/ml), immunoprecipitation (40 micro;g/ml), and immunocytochemistry (2 micro;g/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bA variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest./p