His-6Poly-ubiquitin chains (Ub2-7)
- 貨號 Cat. No.
- UCH-330
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
His-6Poly-ubiquitin chains (Ub2-7)
human recombinant
Product Information
100 μg
> 95% by SDS-PAGE
Aqueous solutions up to 5 mg/ml
This is a lyophilized powder
Use & Storage
Typical concentrations will depend on specific assay conditions and method of detection.
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.
Catalog #: UCH-330
His-6Poly-ubiquitin chains (Ub2-7)
Product Source:
human recombinant
Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys48- linked chains are abundant in vivo and act as a universal signal for proteasomal degradation. This product is formed with His6-tagged wild-type human recombinant ubiquitin and linkage-specific enzymes. This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species; mono-ubiquitin has been removed. The His6-tag is convenient for metal chelate affinity purification and immuno-detection using His6-specific antibodies.
Product Information
Quantity:
100 μg
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 5 mg/ml
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations will depend on specific assay conditions and method of detection.
Storage:
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.