Proteins and Peptides
HIF1A Antibody (LS-B110)
- 貨號 Cat. No.
- LS-B110
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B110 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C2556. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Hypoxia-inducible Factor 1 Alpha (HIF1A) |
| Synonyms: | HIF1A, hypoxia-inducible factor 1 alpha |
| Host: | HIF1A antibody was produced in Mouse |
| Antigen Species: | HIF1A antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG2b |
| Antibody Clone Name: | H1alpha67 |
| Antigen Type: | Fusion protein |
| Immunogen: | HIF1A antibody was raised against fusion protein |
| Antibody Specificity: | Fusion protein containing amino acids 432-528 of human HIF-1alpha. |
| Antigen Modification: | aa432-528 |
| Predicted Species Reactivity: | Bovine, Ferret, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.05% sodium azide. |
| Recommended Storage: | Store at 4°C. Do not freeze. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B110 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1:500), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 4 mg/ml |
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