Proteins and Peptides
HIF1A Antibody (LS-B109)
- 貨號 Cat. No.
- LS-B109
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B109 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C2555. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Hypoxia-inducible Factor 1 Alpha (HIF1A) |
| Synonyms: | HIF1A, hypoxia-inducible factor 1 alpha |
| Host: | HIF1A antibody was produced in Mouse |
| Antigen Species: | HIF1A antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | ESEE122 |
| Antibody Specificity: | Human HIF-1 alpha amino acids 329-530. |
| Antigen Modification: | aa329-530 |
| Predicted Species Reactivity: | Bovine, Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | Buffer, 0.01% sodium azide. |
| Recommended Storage: | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1.25 µg/ml), Immunocytochemistry (ICC), Immunofluorescence (IF) (8 - 12 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 2 mg/ml |
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