HHV-7 H7-4 Strain Quantitated DNA Control

貨號 Cat. No.
08-950-250
規格 Size
250 µl
品牌 Brand
Advanced Biotechnologies Inc

Description







HHV-7H7-4 quantitated DNA PCR control.








Catalog Number: 

08-950-250






Status: 

Current product






Availability: 

Usually ships within two business days






Discount: 

Quantity discounts are available for quantities of 5 or more. Please contact us for details.






Size: 

250 µL






Use: 

For research use only. Not for use in diagnostic procedures.






Storage Temperature: 

-20ËšC




Virus: HHV-7, strain H7-4


Virus Preparation: Purified virus


 Cell Line for Propagation: SupT-1


Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0


DNA Copy Number: 1-2 × 104 copies/µL (varies by lot, please contact ABI for details)


PCR Analysis of DNA Control: PCR analysis was performed on purified viral DNA using the PE Biosystems GeneAmp® Gold PCR Reagent Kit and primers specific for the major capsid protein gene of HHV-7. The reaction produces a 336 bp fragment. The expected band was observed from 105 copies/reaction down to 103 copies/reaction.

DNA Quantitation: Copy number of quantitative viral DNA is determined by real time PCR using the Roche LightCycler®. DNA copy number may vary dependent on the quantitation method used.


Application: ABI’s quantitated DNA controls are prepared from virus, bacteria, parasites, or mollicutes, and are intended for use as positive PCR quantitation standards for the organism in question.  Due to the nature of quantitative DNA controls, ABI cannot guarantee their suitability as extraction controls.  Additionally, due to the extreme sensitivity of detection in PCR reaction, and since no method of purification can guarantee the complete absence of extraneous agents, quantitative DNA controls are not intended for use as negative controls for other organisms.






Shipping and Storage: 

This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.



 






Safe Handling Recommendations: 

This DNA extraction procedure has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.





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