HGF Protein, Human, Recombinant

Purity

> 93 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation.

The ED50 for this effect is typically 2-10 ng/mL.

Protein Construction

A DNA sequence encoding the human HGF (NP_000592.3) precursor (Met 1-Ser 728) was expressed and purified.

NP#

Expressed Host

CHO Stable Cells

Species

Human

Predicted N Terminal

Gln 32 (α chain) & Val 495 (β chain)

Molecule Mass

The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4

1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.