hGDNF

hGDNF

human Glial-cell line Derived Neurotrophic Factor

Product#:

G-240

Sizes:

1 mg

5 x 1 mg

5 mg

10 mg

Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-beta superfamily.  

GDNF signals through a multi-component receptor system, composed of a RET protooncogene  

and one of the four alpha1-alpha4 receptors.¹  



GDNF promotes survival of various neuronal cells, including motoneurons,^2,3 Purkinje cells  

and sympathetic neurons.⁴ In embryonic midbrain cultures, GDNF promotes the survival and  

morphological differentiation of dopaminergic neurons and increases their high-affinity  

dopamine uptake.⁵ Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann  

cells,⁶ neurons, pinealocytes, and skeletal muscle cells.⁷  



In vivo, following transection of facial motor neuron axons, locally applied GDNF has 

been shown to rescue virtually all damaged neurons from death.⁸ GDNF may be of clinical 

relevance in the treatment of Parkinson's disease that is characterized by progressive  

degeneration of midbrain dopaminergic neurons.^9,10

Preparation:

hGDNF is a recombinant growth factor produced in E. coli.

M.W.:

30.1 kDa for the dimer.

Purity:

Greater than 98% as estimated by SDS-PAGE analysis.

Bioassay:

GDNF induces protein kinase B (Akt) and ERK1/2 MAPK (P44/42) activation in SHSY-5Y cells.

SHSY-5Y cells were serum starved for 2h, and then stimulated with different concentrations of hGDNF (#G-240) for 10 min.  The cell proteins were resolved by SDS PAGE and detected with phospho-(Ser 473)-Akt and phospho-ERK1/2 antibody.

Locus Link ID:

2668 GDNF.

References:

1.	Airaksinen, M.S. et al. (2002) Nat. Rev. Neurosci. 3, 383.
2.	Henderson, C.E. et al. (1994) Science 266, 1062.
3.	Houenou, L.J. et al. (1996) Cell. Tissue Res. 286, 219.
4.	Kobayashi, M. et al. (2000) Neuroreport 11, 2541.
5.	Tomac, A. et al. (1995) Nature 373, 335.
6.	Zhou, F.Q. et al. (2003) Cell 113, 814.
7.	Lin, L.F. et al. (1993) Science 260, 1130.
8.	Matheson, C.R. et al. (1997) Neuroreport 8, 1739.
9.	Brundin, P. (2002) Brain 125, 2149.
10.	Grondin, R. et al. (1998) J. Neurol. 245, P35.