Hexokinase (Rat) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of hexokinase in rat serum, plasma, culture supernatants and other biological fluids.

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Rat
• Application- quantitative measurement of hexokinase in rat serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of hexokinase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 31.25 - 2000 pg/ml
Sensitivity: < 18.75 pg/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
No significant cross-reactivity or interference was observed.

Description:

Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. BioVision’s Hexokinase ELISA kit is a sandwich ELISA assay for the quantitative measurement of Hexokinase in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Hexokinase captured from the samples.