Her2 / ERBB2 Protein, Human, Recombinant (His Tag)

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA . Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1.28-32 ng/ml.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.

NP#

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Thr 23

Molecule Mass

The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4

1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.