Hep 3B 2.1-7
- 貨號 Cat. No.
- CLC012
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC012) | |
Designations | Hep 3B 2.1-7 |
| Cell type | Human hepatocellular carcinoma |
Growth property | Adherent |
| Seeding density(cells/ml) | 2.2x106 cells/ml |
survival rate | 91% |
Culture medium | 90% Minnmum essential medium (Eagle) with 2mM L-glutamine and Earle’s BSS adjusted to contain 1.5g/L sodium bicarbonate 0.1mM non-essential amino acids， and 1.0mM sodium pyruvate+10% fetal bovine serum. |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 93% culture medium+7% DMSO |
Medium renewal | Every 2 to 3 days |
| Subculturing |
Remove medium, and rinse with PBS solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
|
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.