Heme Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K672-100
- 規格 Size
- K672-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 growth media and food products 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay
Storage Conditions: 4°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (570 nm)
• Species reactivity- Mammalian
• Application- Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 30 minutes to complete the assay
• Fast and convenient
• Highly sensitive assays for measuring Heme level in biological samples
Description:
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ≈ 650 ng/ml) exerting regulatory functions such as repression of nonspecific δ-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces. BioVision’s Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.