HDAC1 Immunoprecipitation (IP) & Activity Assay Kit

Alternate Name : Histone Deacetylase 1 Immunoprecipitation (IP) & Activity Assay Kit

Tag Line :

Only kit on the market for both IP and measuring activity of HDAC1

Storage Conditions: -20°C

Shelf life: 12 moths

Summary :

• Detection method - Fluorescence (Ex/Em=380/500 nm)
• Applications:
- Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s)
- Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme
- Screening for activators or inhibitors of HDAC1

Detection Method:

Features & Benefits:

• Simple, fast and convenient.
• Only kit on the market for both IP as well as measuring activity of HDAC1

Description:

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.