hCardiotrophin-1

hCardiotrophin-1

 

Product#:

C-200

Sizes:

1 mg

5 x 1 mg

5 mg

Cardiotrophin-1 is a member of the neuropoietic cytokine family (LIF, CNTF, OSM IL-11, IL-6). 

Cardiotrophin-1 is a strong inducer of cardiac hypertrophy, exerts cardio-protective 

effects and plays a role in neuronal growth and differentiation.^1,2 



In neonatal rat, Cardiotrophin-1 has been shown to promote cardiac myocyte survival 

and to enhance embryonic myocyte proliferation during cardiac chamber development.³ 

Cardiotrophin-1 increases angiotensinogen gene transcription and induces VEGF expression 

in cardiac myocytes.^4,5 It also induce vessel growth during cardiac  

remodeling, an effect that may contribute to its cardio-protective properties. 



In humans, a recent report showed an increase in circulating Cardiotrophin-1 in heart  

failure.⁶ A strong correlation between the increased Cardiotrophin-1 and the severity  

of left ventricular systolic dysfunction was found.⁷ 



Cardiotrophin-1 is a very potent neurotrophic factor for motoneurons in long-term  

culture and protects neonatal rat motoneurons from axotomy-induced cell death.^2,8  

It was able to modulate the synthesis of specific neurotransmitters by cultured neuronal cells,⁹  

and to promote the survival of rat dopaminergic and chick ciliary neurons.¹⁰ 

Recently, the potential for neuroprotection by Cardiotrophin-1 was investigated in animal models  

characterized by a prominent axonal degeneration.^11-13 

Preparation:

hCardiotrophin-1 is a recombinant growth factor produced in E. coli.

M.W.:

21.5 kDa

Purity:

Greater than 99% as estimated by SDS-PAGE analysis and HPLC

Bioassay:

The biological activity was tested to confirm the ability of hCardiotrophin-1 to induce p38 MAPK phosphorylation.

3T3-L1 cells were grown to 70% confluence, serum starved for 3 h and then stimulated with various concentrations of hCardiotrophin (#C-200) for 10 min. The cell proteins were resolved by SDS PAGE, probed with Anti-Phospho-p38 MAPK.

Locus Link ID:

1489 cardiotrophin-1.

References:

1.	Pennica, D. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 1142.
2.	Pennica, D. et al. (1998) Neuron 17, 63.
3.	Sheng, Z. et al. (1996) Development 122, 419.
4.	Fukuzawa, J. et al. (2000) Hypertension 35, 1191.
5.	Weiss, T.W. (2003) Cardiovasc. Res. 59, 628.
6.	Talwar, S. et al. (2000) Clin. Sci. (Lond). 99, 83.
7.	Talwar, S. et al. (2002) Clin. Sci. (Lond). 102, 9.
8.	Oppenheim, R.W. et al. (2001) J. Neurosci. 21, 1283.
9.	Cheng, J.G. et al. (1997) J. Neurochem. 69, 2278.
10.	Pennica, D. et al. (1995) J. Biol. Chem. 270, 10915.
11.	Lesbordes, J.C. et al. (2003) Hum. Mol. Genet. 12, 1233.
12.	Zhang, Z.F. et al. (2003) Neurotox. Res. 5, 539.
13.	Bordet, T. et al. (2001) Hum. Mol. Genet. 10, 1925.