HAT (H4) Activity Fluorometric Assay Kit

Alternate Name : Histone Acetyltransferase (H4) Activity Fluorometric Assay Kit

Tag Line :

Sensitive assay to measure HAT activity

Storage Conditions: -80°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em = 535/587 nm)
• Applications- Measurement of HAT activity of immunoprecipitated samples
- Measurement of HAT activity of purified enzyme preparations
- Measurement of HAT activity in Nuclear Extracts.

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

Features & Benefits:

• Simple, rapid & convenient
• The assay can detect HAT Activity equivalent to as low as 1.5 µU of p300 in a variety of samples.

Description:

Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and PicoProbeTM to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples.