HA22T/VGH
- 貨號 Cat. No.
- CLC011
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC011) | |
Designations | HA22T/VGH |
| Cell type | Human hepatocellular carcinoma |
Growth property | Adherent |
| Seeding density(cells/ml) | 2.0x106 |
survival rate | 95% |
Culture medium | 90%DMEM with 2mM L-glutamine and 100uM non-essential amino acids,10%FBS Addittional glutamine and non-essential amino acids |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 95%culture medium+5%DMSO |
Medium renewal | every 3 days |
Subculturing |
Remove medium, and rinse with PBS solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.