HA-Ubiquitin Vinyl Sulfone
- 貨號 Cat. No.
- U-212
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
HA-Ubiquitin Vinyl Sulfone
human recombinant
Product Information
25 μg
8.6 kDa
> 95 % by HPLC.
Soluble in aqueous buffer up to 5 mg/ml. Pellet dry compound prior to reconstitution.
Ki = <40 pM versus UCHL3
Use & Storage
Depending on conditions typical concentrations to fully inhibit DUBs in vitro are 1-5 μM.
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: U-212
HA-Ubiquitin Vinyl Sulfone
Product Source:
human recombinant
Also known as: HA-Ub-VS
This N-terminal HA-tagged ubiquitin is a potent, irreversible and specific inhibitor of most deubiquitinating enzymes (DUBs) including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized be by anti-HA antibodies and/or anti-HA-agarose.
Product Information
Quantity:
25 μg
MW:
8.6 kDa
Purity:
> 95 % by HPLC.
Solubility:
Soluble in aqueous buffer up to 5 mg/ml. Pellet dry compound prior to reconstitution.
Activity:
Ki = <40 pM versus UCHL3
Use & Storage
Use:
Depending on conditions typical concentrations to fully inhibit DUBs in vitro are 1-5 μM.
Storage:
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.