HA-Ubiquitin Aldehyde
- 貨號 Cat. No.
- U-211
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
HA-Ubiquitin Aldehyde
human recombinant
Product Information
50 μg
8.6 kDa
> 95 % by HPLC.
Soluble in aqueous buffer up to 10 mg/ml. Pellet dry compound prior to reconstitution.
Ki = 2.5 nM vs. UCH Isopeptidase-T.
Use & Storage
Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM.
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: U-211
HA-Ubiquitin Aldehyde
Product Source:
human recombinant
Also known as: HA-Ub-H
Ubiquitin-aldehyde is a potent and specific inhibitor of most deubiquitinating enzymes (DUBs) such as ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). It prevents the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. This tagged version contains an N-terminal HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
Product Information
Quantity:
50 μg
MW:
8.6 kDa
Purity:
> 95 % by HPLC.
Solubility:
Soluble in aqueous buffer up to 10 mg/ml. Pellet dry compound prior to reconstitution.
Activity:
Ki = 2.5 nM vs. UCH Isopeptidase-T.
Use & Storage
Use:
Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM.
Storage:
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.