HA-ISG15 Vinyl Sulfone
- 貨號 Cat. No.
- UL-604
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
HA-ISG15 Vinyl Sulfone
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
18.5 kDa
> 90%
Use & Storage
Add directly to in vitro assay from the stock solution. Depending on conditions and detection method, typical concentrations to fully inhibit ISG15 deconjugating enzymes in vitro are 1-5 μM.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-604
HA-ISG15 Vinyl Sulfone
Product Source:
human recombinant
This N-terminal HA-tagged ISG15 is a potent, irreversible and specific inhibitor of ISG15-specific isopeptidases (such as UBP43, Cat # E-600). This protein blocks the hydrolysis of poly-ISG15 chains on substrate proteins in vitro and thus enhances poly-ISG15 chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
MW:
18.5 kDa
Purity:
> 90%
Use & Storage
Use:
Add directly to in vitro assay from the stock solution. Depending on conditions and detection method, typical concentrations to fully inhibit ISG15 deconjugating enzymes in vitro are 1-5 μM.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.