H2AFX Antibody (LS-B1474)

貨號 Cat. No.
LS-B1474
規格 Size
50 µg
品牌 Brand
LSBio

Description





















































































Antibody Catalog ID: LS-B1474
Antibody Validation: This antibody replaces catalog number LS-C34456. It has been validated for use in the following assays: IHC.
Antibody Target: Histone H2a.x (H2AFX)
Synonyms: H2AFX, histone h2a.x
Host: H2AFX antibody was produced in Rabbit
Antigen Species: H2AFX antibody was raised against Human
Clonality: Polyclonal
Isotype: IgG
Antigen Type: Synthetic peptide - KLH conjugated
Immunogen: H2AFX antibody was raised against synthetic peptide
Antibody Specificity: Amino acids surrounding Ser 139 of human H2AFX
Antigen Modification: Ser139
Predicted Species Reactivity: Mouse, Rat
Purification: Immunoaffinity Chromatography
Presentation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recommended Storage: +4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary: Immunohistochemistry: LS-B1474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1474 was determined to be 10 ug/ml.
Antibody Uses: Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1:500 - 1:1000) (Optimal dilution to be determined by the researcher)
Size: 50 µg
Concentration: 1 mg/ml

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