H-89 Dihydrochloride

H-89 dihydrochloride is an inhibitor of PKA and MSK, inhibits cyclic-AMP response element binding protein-mediated MAPK phosphatase-1 induction by lipopolysaccharide. The Ki value is 48 nM for PKA inhibitor. H-89 having a relatively large number of PKA-independent effects which may seriously compromise interpretation of data, and it also inhibits at least 8 other kinases. As we recognized its kinase inhibiting properties, H89 should not be used as the single source of evidence of PKA involvement, it should be used in conjunction with other PKA inhibitors, such as Rp-cAMPS or PKA analogs.