GST-Ubiquitin
- 貨號 Cat. No.
- U-540
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
GST-Ubiquitin
human recombinant
Product Information
1 mg
[GST-Ub] = X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
35 kDa
>95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations for non rate-limiting conjugate formation is 20-100 μM depending on conditions. Supplementation with ubiquitin may be necessary
for best results in forming high molecular weight conjugates.
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.
Catalog #: U-540
GST-Ubiquitin
Product Source:
human recombinant
Fully functional ubiquitin with an N-terminal GST-tag (glutathione S-transferase). GST-Ub allows for glutathione affinity purification of ubiquitinated molecules and allows for immuno- detection of conjugates using GST antibodies. Facilitates the facile visualization of poly-Ub chains due to larger ladder intervals.
Product Information
Quantity:
1 mg
Stock:
[GST-Ub] = X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
MW:
35 kDa
Purity:
>95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations for non rate-limiting conjugate formation is 20-100 μM depending on conditions. Supplementation with ubiquitin may be necessary
for best results in forming high molecular weight conjugates.
Storage:
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.