GST-UbcH5a, Dominant Negative
- 貨號 Cat. No.
- E2-617
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
GST-UbcH5a, Dominant Negative
human recombinant
Product Information
100 μg
[E2] = X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
43 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E2-617
GST-UbcH5a, Dominant Negative
Product Source:
human recombinant
UbcH5 enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins. UbcH5a has 89% and 88% sequence identity with UbcH5b and UbcH5c respectively. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH5a to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions. This protein has an N-terminal GST tag.
Product Information
Quantity:
100 μg
Stock:
[E2] = X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW:
43 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.