GST Tagged Protein Purification Buffer Kit Gst蛋白質純化試劑
- 貨號 Cat. No.
- CG000
- 規格 Size
- 1 kit
- 品牌 Brand
- gene-rl
Description
Gst蛋白質純化試劑
GST Tagged Protein Purification Buffer Kit | |||||||||||||||||||||||||||||||||||||
(Cat. No. CG000 ) Introduction: |  | ||||||||||||||||||||||||||||||||||||
GST fusion proteins are easily purified from bacterial lysates by affinity chromatography using glutathione immobilized to a matrix such as Sepharose. When applied to the affinity medium, fusion proteins bind to the ligand, and impurities are removed by washing with buffer. Fusion proteins are then eluted from the Glutathione Sepharose under mild, non-denaturing conditions that preserve both protein antigenicity and function. The glutathione ligand is coupled via a 10-carbon linker to highly crosslinked 4% agarose. The coupling is optimized to give high binding capacity for GST-tagged proteins and other glutathione binding proteins. The total binding capacity is approximately 10 mg recombinant GST/ml medium. The dynamic binding capacity will vary depending on several factors such as target protein, flow rate etc. If removal of the GST moiety (a naturally occurring protein with Mr 26 000) is required, the tagged protein can be digested with the appropriate sitespecific protease while bound to Glutathione Sepharose or, alternatively, after elution. Cleavage of GST-tagged protein bound to the column/bulk medium eliminates the extra step of separating the released protein from GST, since the GST-tag remains bound. Contents of the kit:
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